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关键词：
Culture of Animal Cells: A Manual of Basic Techni Que and Specialized Applications, Seventh Edition
书目信息
ISBN：
9781118873656
本馆索书号：
中图分类号：
Q95
中文译名：
作者：
R. Ian Freshney
编者：
语种：
英语
出版信息
出版社：
Wiley
出版地：
出版年：
2016
版本：
版本类型：
原版
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卷期：
文献信息
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前言：
摘要：
This is the 7th edition of a textbook first published in 1983.It aims to provide basic instruction in the basic procedures ofcell culture for newcomers to the field, including aseptictechnique, safety and regulatory issues, equipment and materials,media preparation and sterilization, primary culture, propagatedcell lines, characterization and authentication, contamination,cryopreservation, and quantitation. There are also a number ofspecialized protocols some of which have general interest, e.g.cell cloning, 3D culture, scale-up, STR profiling, and some with awith more limited readership, e.g. culture of some specializedcells. Some specialized protocols will be retained in the printedcopy but others will be presented in electronic form only,depending on the anticipated readership.A number of minireviews, some by the author external review andsome by invited authors will be added to give an overview of theapplications of cell culture.New approaches and procedures have become available and new issueshave arisen which require sections of the book to be updated. Theincreasing diversity of the applications of cell culture also needa revision of howcertain topics are presented. The proliferation of specializedtechniques requires that some of these now be presented online toavoid a further increase in size of the book. In addition theintroduction of new topics requires that some of these be presentedin mini-review form. Three reviewing editors have been appointed to advise on recentdevelopments and trends and this will help to reshape the book inline with cutrrent demand.Some new features:There will be a new chapter on cell line authentication with areview of the major issues and appropriate protocols including DNAprofiling (existing) and barcoding (new). Some specialized protocols, e.g. much of chapters 22, 23, and 27,will be removed and made available online (free to those who havepurchased the print copy).This edition will focus more on more generally used techiniques andmake other less used techniques available online. New mini-reviewswill give insight into newer applications. More emphasis will begiven to authentication and problems of misidentification.Illustrations will be updated as required.
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全文链接：
http://www.itextbook.cn/f/book/bookDetail?bookId=87d9bf855d544ea0b415e024ab0b0d16
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