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关键词:
Essentials of Genetic Engineering
书目信息
ISBN:
9781842653906(13位)
中图分类号:
Q7
杜威分类号:
中文译名:
基因工程精要
作者:
S. K. Jain
编者:
语种:
English
出版信息
出版社:
Alpha Science International Limited
出版地:
出版年:
2007
版本:
版本类型:
原版
丛书题名:
卷期:
文献信息
关键词:
Life Sciences
前言:
摘要:
内容简介:
Genetic engineering has emerged as a very powerful technique that has changed the entire gamut of modern biology. Understanding the basic principles underlying the gene cloning, construction of novel genes and expression of genes in heterologous system is essential to develop an expertise in this field. A number of books are available, however, majority of these do not have the entire information at one place. Essentials of Genetic Engineering aims to provide both the principle and the practical details related to various aspects of genetic engineering. The book starts from details of gene structure, expression of genetic information including the transcription and translation and the regulation of these processes, goes to give details about gene cloning including the principle of relevant techniques and finally takes the reader to various prokaryotic and eukaryotic expression systems. Strategies for achieving high level expression of proteins of biomedical importance in biologically active form and their down stream processing have been discussed. The book is written in a simple language and should be useful to students of Biotechnology, Biochemistry and Life Sciences at the Undergraduate and Postgraduate levels.
目次:
Introduction / Genetic Information and its Transfer / Gene Structure, Gene Expression and Regulation of Gene Expression / Enzymes Involved in Genetic Engineering / Gene Libraries: cDNA vs Genomic Libraries / Cloning Vectors / Cloning Strategies / PCR Assisted Gene Cloning / Screening of Gene Libraries and Isolation of Clones / Characterization of Gene Clones / Analysis of Genes / Expression of Cloned Genes / Bacterial System for Expression / Expression in Yeast / High Level Expression of Foreign Proteins in Insect Cells / Mammalian Cells for Expression of Complex Protein / Down Stream Processing and Purification of Recombinant Protein / Principles and Protocols for Research Techniques used in Genetic Engineering.
附录:
全文链接:
读者对象:
Undergraduate & Postgraduate Students in Biotechnology, Biochemistry and Life Sciences
实体信息
页码:
装帧:
Hardback
尺寸:
其它形态细节:
其它信息
原价:
GBP
49.9500
原版ISBN:
其它ISBN:
图书特色:
书评:
扩展信息
Isbn:
1842653903
issue:
2006JC02
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