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关键词:
Gene Cloning and Manipulation
书目信息
ISBN:
9780521817936(13位)
中图分类号:
Q7
杜威分类号:
中文译名:
基因克隆与操作
作者:
Christopher Howe
编者:
语种:
English
出版信息
出版社:
Cambridge University Press
出版地:
出版年:
2007
版本:
2e
版本类型:
原版
丛书题名:
卷期:
文献信息
关键词:
genetics, biotechnology, biochemistry
前言:
摘要:
内容简介:
Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
目次:
Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples.
附录:
全文链接:
读者对象:
undergraduate students, graduate students
实体信息
页码:
280
装帧:
Hardback
尺寸:
其它形态细节:
其它信息
原价:
USD
120.0000
原版ISBN:
其它ISBN:
图书特色:
书评:
扩展信息
Isbn:
0521817935
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